Table 1.
Hematologic indices of wild-type (+/+), heterozygous (+/ld), and loop-deleted (ld/ld) mice and quantitative-RT-PCR analysis of Slc4a1 mRNA levels in +/+, +/ld, and loop-deleted (ld/ld) mutant mouse tissues
| Values | +/+ | +/ld | ld/ld |
|---|---|---|---|
| Hematologic indices | |||
| WBCs, × 103/μl | 7.68 ± 0.23 | 6.85 ± 1.88 | 5.67 ± 1.3 |
| Platelets, × 103/μl | 917.67 ± 27.58 | 781.14 ± 89.3 | 829.33 ± 134.86 |
| RBCs, × 106/μl | 9.28 ± 0.86 | 8.9 ± 0.46 | 8.75 ± 0.45 |
| Hematocrit, % | 51.2 ± 0.97 | 50.87 ± 1.63 | 50.52 ± 1.38 |
| Hb, g/dl | 15.3 ± 0.57 | 13.8 ± 0.49 | 13.32 ± 0.55 |
| Mean cell volume, fl | 55.17 ± 2.19 | 57.15 ± 1.49 | 57.73 ± 1.14 |
| Ter119+ cells in bone marrow, % | 35.42 ± 3.42 | 37.06 ± 2.53 | 54.66 ± 2.90** |
| Ter119+ cells in spleen, % | 12.86 ± 1.67 | 13.01 ± 3.10 | 23.42 ± 4.86** |
| Reticulocytes, % | 2.18 ± 1.22 | 5.47 ± 1.84* | 13.87 ± 4.42*** |
| QPCR | |||
| Bone marrow | 1.0 | 0.92 ± 0.14 | 1.6 ± 0.25**** |
| Spleen | 0.05 ± 0.02 | 0.05 ± 0.03 | 0.081 ± 0.02**** |
| Kidney | 0.002 ± 0.001 | 0.005 ± 0.003 | 0.006 ± 0.003 |
The level of Slc4a1 mRNA in +/+ bone marrow was arbitrarily designated as 1.0. *, P < 0.04; **, P < 0.002; ***, P < 0.001; ****, P < 0.02.