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. Author manuscript; available in PMC: 2007 Aug 22.
Published in final edited form as: Dev Biol. 2006 Dec 9;303(2):715–726. doi: 10.1016/j.ydbio.2006.12.011

Figure 4. Elevated serum FSH in TAF4b-null mice.

Figure 4

A. Circulating levels of FSH, prolactin, and luteinizing hormone were measured in TAF4b-null mice and in control littermates. Hormonal levels in TAF4b-null mice significantly different from controls are indicated with an asterisk (p<0.001). B-E. Real-time PCR detects normal levels of estrogen receptor beta (Esr2) and FSH receptor (Fshr) mRNAs in TAF4b-null ovarian samples at 3 weeks of age (B, D). At 17 weeks of age, both Esr2 and Fshr mRNA levels are dramatically reduced in TAF4b-null mice (C, E). 18S ribosomal RNA levels were used as a control for normalizing the data. Quantitative real-time PCR was performed in triplicate on the total RNA samples from the ovaries of the wild-type, heterozygous, and TAF4b-null mice at 3 weeks (B, D) or 17 weeks (C, E) of age. The Ct values for Esr2 (B, C) and Fshr (D, E) were normalized to 18S rRNA (ΔCt), and the resultant value was used to calculate the relative expression level. Wild-type expression level values in each experiment were arbitrarily set to 1, and the remaining values were scaled accordingly. Error bars represent 1 SD. 3 week old wild type, TAF4b-null and Het #1 were primed with E2 for 24 hrs. 3 week old Het #2 and all of 17 week old mice were non-treated.