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. 2007 Aug 24;371(4):855–872. doi: 10.1016/j.jmb.2007.06.013

Figure 5.

Figure 5

Rho-mediated RNA release from the stalled elongation complex. (a) Cartoon showing the design of a stalled elongation complex using lac repressor as a roadblock. In this set-up, the EC was stalled at 161 position of the trp t' terminator. Rho was added to the reaction after the stalled complex is formed and RNA release was measured from the supernatant. (b) Autoradiogram showing the amount of released RNA in the supernatant by WT and different Rho mutants both in the absence and presence of 200 nM NusG from the stalled EC. RB and RO denote the positions of roadblocked product and run-off product, respectively. The meaning of S and P is same as described for Figure 1. (c) The RNA release efficiencies shown by bar diagrams are calculated as: [2S]/[S+P].