Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Fluorescence anisotropy (r) values of Tb-GTP
| Species | Anisotropy (r) |
|---|---|
| Free Tb-GTP [150 μM: 50 μM] | 0.096 ± .005 |
| Tb-GTP+ 100 nM WT Rho | 0.246 ± .007 |
| Tb-GTP+ 150 nM Y80C Rho | 0.183 ± .003 |
In all the experiments, Tb-GTP complexes were made by incubating 150 μM Tb with 50 μM GTP. The majority of the Tb-GTP species were in bound form in the presence of the indicated amount of hexameric Rho. Anisotropies were measured at 25 °C.
