FIGURE 4.

Colocalization of endogenously expressed HuR and AUF1. Immunocytochemistry was used to detect the intracellular location of endogenously expressed HuR and AUF1 proteins in DDT1-MF2 cells. (A) In control cells, endogenous HuR (Alexa Fluor 488 conjugated 2° antibody) and AUF1 (Cy3 conjugated 2° antibody) are expressed almost exclusively in the nucleus and are shown to colocalize. (B) Anisomycin treatment induces shuttling of endogenous AUF1 and HuR from the nucleus to the cytoplasm (cf. t=0 h and t=24 h). The merged view shows the colocalization of HuR and AUF1 in the nucleus and the cytoplasm after 24 h of anisomycin treatment. The scale bars in the merged view apply to all images in the same row.