FIGURE 8.
Toeprinting analysis of mRNA translocation catalyzed by EF-G in the presence of different nucleotides and antibiotics. The pre-translocation complex was assembled by binding deacylated tRNAMet (P site), N-Ac-Phe-tRNAPhe (A site), and m291 mRNA to the ribosome. The pre-translocation complex was incubated with EF-G in the presence of GTP, GDPNP, GDP, and fusidic acid as indicated. The toeprint bands at +16 and +19 correspond to the pre- and post-translocation states of the ribosome, respectively.