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. Author manuscript; available in PMC: 2007 Aug 23.
Published in final edited form as: Int Immunol. 2005 Sep 26;17(11):1409–1418. doi: 10.1093/intimm/dxh319

Fig. 2.

Fig. 2

Stimulatory properties of larval released products are independent of endotoxin yet have both TLR4-dependent and -independent components. (A, C) tMφ and (B, D) IFNγ-activated tMφ from C3H/HeN or C3H/HeJ mice were cultured overnight with media alone, 0-3hRP (50 μg ml-1) or LPS (0.001 μg ml-1), in the presence of 0 (black bars) or 10 (white bars) μg ml-1 PMB. In these assays, 0-3hRP contributed the equivalent of 0.0005 μg ml-1 endotoxin to the cell culture. Supernatants from triplicate wells were pooled and analysed by ELISA for the production of IL-6, IL-10 and IL-12p40. Data are the mean ± SD of two experiments. Dotted lines represent the lower limit of ELISA detection.