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. Author manuscript; available in PMC: 2007 Aug 23.
Published in final edited form as: Int Immunol. 2005 Sep 26;17(11):1409–1418. doi: 10.1093/intimm/dxh319

Fig. 4.

Fig. 4

Differential requirement for TLR4 and MyD88 in the signalling pathway stimulated by 0-3hRP. (A) tMφ from C57Bl/6 (open bars) and TLR4-/- (black bars) mice or (B) C57Bl/6 (open bars) and MyD88-/- (hatched bars) mice were cultured overnight with media alone, 0-3hRP (50 μg ml-1) plus PMB (3 μg ml-1), LPS (0.01 μg ml-1) or Zymosan A (5 μg ml-1). Supernatants were removed and analysed by ELISA for the production of IL-12p40 and IL-6. (A) Cytokine production is displayed as a percentage of the amount produced by the respective cells when cultured with Zymosan A. (B) Alternatively, cytokine production is displayed as the actual concentration detected in the culture supernatants. Data are presented as the mean ± SD of three wells.