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. Author manuscript; available in PMC: 2007 Aug 23.
Published in final edited form as: Biol Reprod. 2004 May 5;71(3):828–836. doi: 10.1095/biolreprod.104.030569

FIG. 5.

FIG. 5

Fanca is expressed in adult mouse pituitary. A) RT-PCR analysis of RNA extracted from adult mouse pituitary was performed using primers that amplified exons 7-18, exons 14-18, and exons 30-32 of mouse Fanca. Ethidium bromide staining identified PCR products for all regions amplified. A control GAPDH PCR product was also amplified, confirming the integrity of the mouse pituitary cDNA. B) RNA was extracted from LβT2, αT3-1, HeLa, and L cells; reverse transcribed; and first-strand cDNA was analyzed by PCR for expression of Fanca. The PCR primers corresponded to exons 7-18 of mouse Fanca cDNA and ethidium bromide staining identified a 450-bp product in LβT2, HeLa, and L cells. No Fanca expression was detectable when amplifying αT3-1 cDNA. In this experiment, expression of Zfp276 was used as an internal control to check PCR conditions and RNA integrity. A specific 1238-bp PCR product, corresponding to Zfp276, was amplified from LβT2, αT3-1, and L cell first-strand cDNA.