TABLE 2.

Specific primers were used in PCR reactions to amplify first strand Fanca, Zfp276, and B-2-microglobulin cDNA.

Specific primers*	DNA sequence	Location	PCR product (base pairs)
Fanca s	5′-CTGTGTGAGCAGATAGGC-3′	Exon 7 (640 bp)	979
Fanca as	5′-TCACGCTCGGCAATGTCCC-3′	Exon 17 (1619 bp)
Fanca s	5′-CAGCATGGTCACTGCGTTCC-3′	Exon 14 (1263 bp)	450
Fanca as	5′-CCTGAATATGCTGGCCTCCA-3′	Exon 18 (1713 bp)
Fanca s	5′-GTGGTGGAGACCTGGAAGA-3′	Exon 30 (2900 bp)	211
Fanca as	5′-CGGCGTAGAACAGCCATG-3′	Exon 32 (3111 bp)
Fanca s	5′-GCACTTTGCGTGGAGAGG-3′	Exon 37 (3666 bp)	129
Fanca as	5′-CAGGTAGGACGAGAGTAGAC-3′	Exon 38 (3795 bp)
Zfp276 s	5′-CACTGTCCTCTGAGTACTGC-3′	5′ ATG 68 bp	1238
Zfp276 as	5′-CGTCACCTGCTGAGTTCAAG-3′	3′ ATG 1170 bp
B2m s	5′-ATGGCTCGCTCGGTGACCCTGGT-3′	Exon 1 (ATG)	102
B2m as	5′-TGTTCGGCTTCCCATTCTCC-3′	Exon 1 (102 bp)

^*Primer annealing sites are expressed relative to the translational start site (ATG) in Fanca, Zfp276, and B-2-microglobulin (B2m). s, Sense; as, antisense; bp, base pairs.