Table 3. .
PCR Primers for Mutation Screening and Transcript Analysis of CHMP4B
| Primer | Location | Strand | Sequence (5′→3′)a |
| Ex1F | Exon 1 | Sense | gtagtcagtggcgcgttg |
| Ex1R | Intron 1 | Antisense | aggcgagtctgatgaaggtg |
| Ex2F | Intron 1 | Sense | cactagaacctcaccctgtgc |
| Ex2R | Intron 2 | Antisense | aaacaaactcaggtgctcgaa |
| Ex3F | Intron 2 | Sense | tcacagggagtcattgcaggg |
| Ex3R | Intron 3 | Antisense | cccaccctggaaaggtgcag |
| Ex3R2 | Intron 3 | Antisense | agggacagcctcagggtatcattt |
| Ex4F | Intron 3 | Sense | cacagggtctggaacctggaa |
| Ex4R | Intron 4 | Antisense | tgggcaagctcaggacacaga |
| Ex5F1 | Intron 4 | Sense | aacatgttgaacgcaccagtc |
| Ex5R1 | Exon 5 | Antisense | AGGTCATTTCAACTGCAACCA |
| Ex5F2 | Exon 5 | Sense | CGCTGACTCCACTGCTGAATCC |
| Ex5R2 | Exon 5 | Antisense | ctggaaagggtcagctcccg |
| StartF | Exon 1 | Sense | caccATGTCGGTGTTCGGGAAGCT |
| EndR | Exon 5 | Antisense | CATGGATCCAGCCCAGTTCTCCAA |
| A-alleleR | Exon 3 | Antisense | CAGCAATGTCCTGCATTAACTCAT |
| T-alleleR | Exon 3 | Antisense | CAGCAATGTCCTGCATTAACTCAA |
a
Noncoding sequence is shown in lowercase, coding sequence in uppercase.