Figure 1. .

Release of ER-retained Tr-J and Tr mutants with curcumin treatment. A, Subcellular localization of wild-type (Wt) PMP22, Tr-J, and Tr in transiently transfected HeLa cells. Tr-J and Tr are extensively retained in the ER, as evidenced by colocalization with PDI (arrows in f and i). B, Curcumin treatment of HeLa cells rescues Tr-J and Tr mutants (green) retained in the ER (PDI markers [red]), as revealed by increased cytoplasmic staining. Cells treated with 40 μM curcumin showed reduced ER retention (arrows in f and i). Scale bars = 25 μm.