Figure 4. .

Improved neuromotor behavior in treated Tr-J mice. A, Rotarod test performed with 3-mo-old wild-type (Wt), Tr-J, curcumin-treated Tr-J, and placebo-treated mice. In three series and 10 trials, the time that animals remained on a rod was measured and plotted. The rotation speed was increased every minute, from 16 to 36 rpm, in steps of 4 rpm. The mean holding time of curcumin-treated Tr-J mice (n=20) was significantly higher than that of placebo and Tr-J mice (n=10). All animals were allowed to stay on the rod for a maximum of 270 s. B, Curcumin treatment was discontinued for a group of Tr-J mice (n=5) after 3 mo of treatment. The rotarod test was performed similar to as described above, after curcumin treatment followed by discontinuation of treatment (marked on the X-axis with an asterisk [*]). We did not observe significant difference in the motor performance of Tr-J mice at 1 wk (trials 12–14), 2 wk (trials 15–18), and 1 mo (trials 19–22) after withdrawal of curcumin. Tr-J mice started to weaken significantly at 2 mo after treatment was ceased (trials 23–26) (P<.001). A double asterisk (**) denotes the group of mice (n=5) that were originally treated with curcumin for 3 mo and then had the treatment discontinued.