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. Author manuscript; available in PMC: 2008 Apr 15.
Published in final edited form as: Dev Biol. 2007 Jan 19;304(2):713–721. doi: 10.1016/j.ydbio.2007.01.021

Fig. 1. Pax6 is expressed in the adult ciliary epithelia and RSC spheres.

Fig. 1

A. confocal image of Pax6 immunostaining of a ciliary process; Anti-Pax6 Monoclonal antibody stains the nuclei of the positive cells. White arrowheads show the less intensely stained the ciliary epithelial cells of the inner layer; black arrows indicate the more intensely stained ciliary epithelial cells in the outer layer. The arrowheads and arrows mark the epithelial cells on one side of the ciliary process. B. RT-PCR of Pax6 gene expression. Pax6 is detected in clonally derived RSC spheres (RSC) and ciliary epithelia (CE) of adult mice. N ctl: negative control (amplification without reverse transcription). Amplicons encompass the alternatively spliced exon 5a (Ton et al., 1991; Walther and Gruss, 1991). The amplification products with or without alternatively spliced exon 5a were labeled as +5a and -5a. C. The ratio of the +5a and −5a transcripts normalized by the control housekeeping gene GAPDH.