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. Author manuscript; available in PMC: 2008 Apr 15.
Published in final edited form as: Dev Biol. 2007 Jan 19;304(2):713–721. doi: 10.1016/j.ydbio.2007.01.021

Fig. 3. Pax6 is required for RSC sphere formation in adult animals.

Fig. 3

A. RSC sphere assays in the Pax6 conditional knockout mouse model (Marquardt et al., 2001). ko: α-Cre +/-; Pax6loxP/loxP (n=4). het: α-Cre +/-; Pax6loxP/wt (n=4). wt: α-Cre -/-; Pax6wt/wt (n=4). B. whole mount fluorescent images of adult α-Cre, Z/EG mouse eyes showing the α-promoter-driven expression has gaps (marked by white brackets) as seen from dorsal (left) and ventral (right) views of the retina. Anterior is on the left. C. RT-PCR of Pax6 on the clonal RSC spheres derived from adult α-Cre; Pax6loxP/loxP ciliary epithelial cells. The same primers, encompassing the alternatively spliced exon 5a were used. 1: wild type single RSC sphere; 2-6: RSC spheres derived from α-Cre; Pax6loxP/loxP CE cells, 7: negative control (amplification without reverse transcription).