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. 2007 Sep 1;21(17):2150–2160. doi: 10.1101/gad.1583807

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Copyright © 2007, Cold Spring Harbor Laboratory Press

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Figure 3. — tT(AGU)C establishes cohesion of HMR. (A) Colocalization of extended HMR circles produced by the G1 and M excision protocols using strains RDY152 (wt) and RDY180 [Δtt(agu)c∷loxP]. (B) Colocalization of the unexcised chromatids in strains RDY152 (wt), RDY180 [Δtt(agu)c∷loxP], and RDY209 (Δbrf1 with plasmid pbrf1-II.9, grown at 24°C). Cultures were supplemented with dextrose rather than galactose in the M excision protocol. (C) Colocalization of simple HMR circles requires tT(AGU)C. Circles were produced by the M excision protocol in strains RDY143 (wt) and RDY279 [Δtt(agu)c∷loxP]. (D) Colocalization of extended HMR circles does not require GIT1. Circles were produced by the M excision protocol using strains RDY152 (wt) and RDY226 (Δgit1∷klURA3).

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