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Southern blot of nestin PCR product (490 bp, arrowhead) followed by hybridization with [32P]dCTP-radiolabeled human nestin cDNA as a probe. Hybridization was detected in three giant cells (G; lanes 1, 2, and 4), a TSC neuron (T; lane 6), and a control neuron (N; lane 3). A 490-bp PCR product was not detected in one giant cell (lane 5).
