Figure 5. .

Estimation of tumor populations carrying 9p UPD and the JAK2 mutation in MPD samples. The populations of 9p UPD–positive components in the 53 MPD cases were estimated by calculation of the mean difference of AsCNs within the UPD regions. Heterozygous (blue bars) or homozygous (red bars) JAK2 mutations in MPD samples were also estimated by measurement of JAK2 mutated alleles and UPD alleles, under the assumption that all the UPD alleles have a JAK2 mutation. Measurement of JAK2 mutated alleles was performed by allele-specific PCR. For three cases having trisomy components (orange bars), the duplicated allele was assumed to have a JAK2 mutation, which is the consistent interpretation of the observed fraction of trisomy and mutated JAK2 alleles for case PV_02 (table 2). mt = JAK2 mutated allele; wt = wild-type allele.