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Expression of APP mRNA in choroid plexus tissues and Z310 cells by real-time RT-PCR. Following PCR, the double-stranded products were separated by gel electrophoresis and visualized with ethidium bromide. Upper: APP produced a double stranded product of 86 base pairs (bp). Lower: GAPDH produced a double stranded product of 82 bp. Lanes 1–5 used cDNA generated from Z310 cells. Lanes 6 and 7 are choroid plexus cDNAs.
