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. Author manuscript; available in PMC: 2007 Nov 1.
Published in final edited form as: Dev Biol. 2006 Jul 21;299(1):137–150. doi: 10.1016/j.ydbio.2006.07.016

Figure 3. Neural crest cells migrate faster toward noggin producing cells.

Figure 3

300–400 μm of E11.5 mouse midgut was cultured in collagen gel with (A, B) or without (C) GDNF (200 ng/mL). (A) Control or (B) noggin secreting CHO cells were placed 400 μm away from the anti-mesenteric border of the explant edge and cultures were maintained for 48 hours. (D) The distance that neural crest derived cells migrated toward (proximal) or away (distal) from the CHO cells was measured. With control CHO cells, proximal and distal migration distances were equal. In contrast, cells migrated a greater distance on the side of the gut with noggin producing CHO cells. (C) Migration out of the gut in response to noggin alone (i.e., without GDNF) was minimal. Scale bar = 100 μm