Figure 9. PSA removal with endo-N prevents BMP induced delays in NCC migration through the colon.
(A–D) E11.5 gut explants were maintained in culture for 48 hours (A) under control conditions, or with (B) endo-N, (C) BMP4, or (D) BMP4 plus endo-N. Endo-N did not affect NCC migration under control conditions, but prevented the delay in NCC migration induced by BMP4. White lines indicate the end of the colon. Ce = cecum. (E) Quantitative analysis of colon innervation (n = 10 explants for each treatment). Scale bar = 100 μm. Landmarks for these measurements are in Figure 4F.