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. 2007 Aug 24;3(8):e163. doi: 10.1371/journal.pcbi.0030163

Figure 3. In Vitro Analysis of Model Predictions.

Figure 3

(A) Activation of ERK and JNK upon CD28, TCR (CD3), or TCR + CD28 stimulation in mouse splenic T cells.

(B) Activation of PKB upon TCR, TCR+ CD4, and TCR + CD28 stimulation in Fyn-deficient and heterozygous splenic mouse T cells.

(C) Inhibition of PI3K with both Ly294002 and Wortmannin blocks the phosphorylation of PKB, ERK, and JNK, but not ZAP-70 in human T cells.

(D) Inhibition of PI3K with both Ly294002 and Wortmannin blocks the phosphorylation of PKB, but not of JNK in human T cells upon CD28 stimulation. As a control, the total amount of ZAP70 (A) or β-actin (B–D) was determined. One representative experiment (of three) is shown.