TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| B. subtilis WB700 | B. subtilis 168 trpC2 ΔnprE ΔaprE Δepr Δbpf Δmpr ΔnprB ΔvrpE Eryr Linr | 35 |
| B. subtilis CH1 | WB700 prototroph | This study |
| B. subtilis CH1 ΔalsS mutant | CH1 als::Spt | This study |
| B. subtilis BS35 | CH1 ldh::pdc-adhB Cmr | This study |
| B. subtilis BS36 | CH1 ldh::pdc-adhB Cmrals::Spt | This study |
| B. subtilis BS37 | CH1 ldh::pdc-adhB Cmrals::udhA_Spt | This study |
| Plasmids | ||
| pUC19 | pMB1 ori Apr | BioLabs |
| pCR-Blunt II-TOPO | pBR322 ori KmrccdB selection | Invitrogen |
| pLOI276 | pUC19 carrying pdcZm | 5 |
| pLOI284 | pUC19 carrying adhBZm | 6 |
| IpTOPO-pdc | pCR carrying pdcZm | This study |
| pTOPO-adh-term | pCR carrying adhBZm-cryIIIAt | This study |
| pTOPO-pdc-adh-term | pCR carrying pdcZm, adhBZm, and Cmr | This study |
| pSG-PLK | pBR322 ori Apr | 20 |
| pSG-operon | pSG-PLK carrying pdcZm, adhBZm, and Cmr | This study |
| pUC-ldh | pUC19 carrying ldhBs | This study |
| pUC-ldh::operonCm | pUC19 carrying ldhBs::(pdcZmadhBZm) | This study |
| pTOPO-alsS | pCR carrying alsS | This study |
| pCm::Spt | pIC177 carrying Cmr::Sptr | 30 |
| pTOPO-alsS::Spt | pCR carrying alsS::Sptr | This study |
| pTOPO-alsS::udhA_Spt | pCR carrying alsS::(udhAEc Sptr) | This study |
a
Zm, Z. mobilis; Bs, B. subtilis; Ec, E. coli.