Scheme of the recombinant plasmid pEAAC. Numbers indicate the positions of relevant sequences. The length of the plasmid is indicated in base pairs. Abbreviations: apr, ampicillin resistance gene; tsr, thiostrepton resistance gene; pemrE*, ermE* promoter; RBS, ribosome binding site; GTG, start codon; ori pUC, replication origin from pUC plasmid; ori pWHM4, replication origin from pWHM4 plasmid.