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Kinetic parameters of recombinant AuAAC assayed with different natural penicillins
| Substrate | Km (mM) | kcat (s−1) | kcat/Km (mM−1 s−1) |
|---|---|---|---|
| Penicillin V | 15.4 | 70.3 | 4.55 |
| Penicillin K | 1.0 | 33.3 | 34.79 |
| Penicillin dihydroF | 5.6 | 4.2 | 0.75 |
| Penicillin F | 15.1 | 1.9 | 0.13 |
| Penicillin G | 155.8 | 2.2 | 0.01 |
