TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| P. naphthalenivorans | ||
| CJ2 | Naphthalene degrader | 11 |
| CJM110 | P. naphthalenivorans CJ2 ΔnagR2::Km lacZ+ | 12 |
| CJM112 | P. naphthalenivorans CJ2 nagR2-nagX::lacZ+ | 12 |
| CJ2ΔnagXKm | P. naphthalenivorans CJ2 ΔnagX::Km lacZ+sacB | This study |
| CJ2ΔnagX | P. naphthalenivorans CJ2 ΔnagX | This study |
| E. coli | ||
| S17-1/λpir | Carries RK2 tra regulon and pir; host for pir-dependent plasmids | 15 |
| BL21(DE3) | F−dcm ompT hsdS(rB− mB−) gal λ (DE3) | Novagen |
| Plasmids | ||
| pET21b | Expression vector; Apr; carries T7 tag, multiple cloning site, His tag | Novagen |
| pBluescript II SK(−) | Cloning vector; carries multiple cloning site in lacZα; Apr | Stratagene |
| pK19mobsacB | Integration vector; Kmr oriVEc oriT sacB | 25 |
| pET21bnagX | NdeI-BamHI-cut PCR fragment containing nagX inserted into pET21b | This study |
| pBSIInagX | 1,072-bp XbaI-SalI-cut PCR fragment containing nagX inserted into pBluescript II SK(−) | This study |
| pBSIIΔnagX | Carries deletion of the 14-bp fragment of nagX from pBSIInagX | This study |
| pK19mobsacBΔnagX | Carries insertion of the 1,058-bp XbaI-SalI insert from pBSIIΔnagX into pK19mobsacB | This study |