TABLE 1.
Time (h) | No UV treatment
|
Half UV dosage
|
Full UV dosage
|
|||
---|---|---|---|---|---|---|
Avg count g−1 | Avg viability (%) | Avg count g−1 | Avg viability (%) | Avg count g−1 | Avg viability (%) | |
0 | 46.0 ± 1.7 | 73.0 ± 5.2 | 46.5 ± 2.1 | 71.5 ± 6.4 | 47.7 ± 2.5 | 71.7 ± 4.5 |
48 | 14.0 ± 2.6 | 53.7 ± 2.5 | 11.0 ± 0.0 | 32.5 ± 0.7 | 14.7 ± 1.2 | 17.6 ± 3.7 |
Oocyst counts and viability were measured from oysters prior to and after 48 h of depuration at both full (standard) and half-power UV treatments. Each assay was performed in triplicate, and oocyst numbers and viability were assessed using standard immunofluorescent antibody and DAPI/PI staining with microscopic examination at magnifications of ×40 and ×100. Standard deviations were calculated using three separate oocyst counts from each trial, which were each performed in triplicate. UV dosage was administered according to United Kingdom guidelines for standard depuration.