TABLE 2.
Survival of C. parvum oocysts recovered from the circulation water of experimentally contaminated oystersa
| Time (h) | No UV treatment
|
Full UV dosage
|
||
|---|---|---|---|---|
| Avg viability (%) | Avg total oocyst count liter−1 | Avg viability (%) | Avg total oocyst count liter−1 | |
| 24 | 71.8 ± 2.3 | 51 ± 5.6 | 15.7 ± 2.5 | 17.3 ± 11.8 |
| 48 | 63.9 ± 8.0 | 922 ± 64.0 | 3.2 ± 4.0 | 766.7 ± 520.4 |
a
Total oocyst count and viability in circulation water with experimentally contaminated oysters during (24 h) and after (48 h) standard UV depuration were determined using standard immunofluorescent antibody and DAPI/PI staining, with microscopic examination at magnifications of ×40 and ×100. Each assay was performed in triplicate, and standard deviations were calculated on the basis of three separate oocyst counts. Values represent the average number of oocysts recovered per liter of circulation water. UV dosage was administered according to United Kingdom guidelines for standard depuration.