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Growth of P. pseudoalcaligenes CECT5344 (A) and the CIO1 mutant (B) in minimal media. Cells were precultured in 2 mM ammonium media until nitrogen was exhausted (OD600 ≈ 0.3). Arrows indicate the addition of 1 mM of nitrogen source in the form of ammonium (•), cyanide from jewelry residue (○), or sodium cyanide (▾). One flask was kept without N addition as a control of nitrogen-free medium (▵).
