FIG. 2.

Effect of cyanide, potassium, and sodium in the respiratory activity of P. pseudoalcaligenes CECT5344 membrane cell extracts. Cells were precultured in 2 mM ammonium medium until nitrogen was exhausted, and after 4 h of growth with 2 mM of cyanide from jewelry residue, the membrane fractions were obtained by ultracentrifugation. NADH oxidation activities were determined as described in Materials and Methods with the additions indicated. The bars represent the average of three independent experiments.