FIG. 1.
Str dependence of the rpsL1 mutant and its single mutant derivatives. Overnight cultures of the wild-type strain grown on TB and of the rpsL1, K47R, and K57E mutants grown with 100 mg/liter of Str in the same medium were diluted (10−5 and 10−6) and plated (100 μl) on TB plates with increasing Str concentrations. After 48 h at 70°C, the number of colonies that grew in each plate was counted and is represented as the percentage of viable cells with respect to their respective maximum values. The data represent the mean values and the standard deviations of two independent assays. The y-axis minimum value (0.2%) is limited by the sensitivity of the assays.