FIG. 5.

Phenotypic analysis of the ctnA disruptant and the ctnA-complemented ctnA disruptant. (A) Growth in liquid culture. Growth was expressed by the weight of dry mycelia for the wild-type strain (diamonds), the ctnA disruptant (squares), and the ctnA-complemented ctnA disruptant (triangles). (B) Pigment production. Pigments were extracted from dry mycelia with 70% ethanol, and the optical density at 500 nm (OD₅₀₀) was measured for the wild-type strain (diamonds) and the ctnA disruptant (squares). (C) Comparison of CT production among the wild-type strain (diamonds), the ctnA disruptant (squares), and the ctnA-complemented ctnA disruptant (triangles). The CT contents in both the mycelia and mycelium-free filtrate were measured by high-performance liquid chromatography and enzyme-linked immunosorbent assay, and the sum is shown. These experiments were performed at least three times independently.