TABLE 1.
Primers used in this study
| Primera | Sequence (5′ to 3′)b |
|---|---|
| act F | GGAATTCTGCAGATTCTACAACGAACTCCG |
| act R | GGAATTCTGCAGTCAGGGAGTTCATAGGAC |
| amp F | GTAGATAACTACGATACGGG |
| amp R | TATGTGGCGCGGTATTATCC |
| hph F | GGGGTACCCTTCTGATCGAAAAGTTCGAC |
| hph R | GGGGTACCCCTCTGATAGAGTTGGTCAAG |
| dehy F | TAGCATCTACTCCAGCGTGA |
| dehy R | TCGGGTAAAGATCTTTGTGC |
| reg F | AAACTACGCTGTGACGGACA |
| reg R | TAACTGCACCAGACGAAACG |
| oxg F | TGCACCTCTACAGGGTTATT |
| oxg R | TCTGCTCATCATATCTCCA |
| oxr F | TAATGCTGCCATCTTCCAAG |
| oxr R | ATACGCAACGAGCGAGACAT |
| pksCT F | CCAGTGTGGCTATTCACC |
| pksCT R | CCTAAGGACATTACTCTT |
| tra F | TGACCATCTGGGTAACCTTC |
| tra R | GAGATGAGTCGACGATGAAG |
| dis F | ACACGGAAGAGATCCAAGCA |
| dis R | AGAGATGAATCACACTCGCC |
a
The forward (F) and reverse (R) primer pairs act, amp, hph, dehy, reg, oxg, oxr, pksCT, and tra were used in RT-PCR for amplification of the actin gene, ampicillin resistance gene, hygromycin resistance gene, dehydrogenase gene (orf1), regulator gene (ctnA), oxygenase gene (orf3), oxidoreductase gene (orf4), polyketide synthase gene (pksCT), and transporter gene (orf5), respectively.
b
Underlining indicates EcoRI and PstI restriction sites.