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. 2007 Jun 8;73(16):5183–5189. doi: 10.1128/AEM.02776-06

FIG. 1.

FIG. 1.

Specificity of primers for V. parahaemolyticus used in real-time RT-PCR. PCR products were hybridized with digoxigenin-labeled probes P2-rpoS (A) and P3-tdh2 (B) specific for rpoS and tdh2 gene amplicons, respectively. The specificity was tested with various strains of V. parahaemolyticus, the tdh1+ tdh2+ (positions 1 to 4), tdh2+ trh+ (positions 5 and 6), tdh1 tdh2 trh negative (position 7), and trh+ (positions 8 to 14) strains, and strains of V. cholerae (position 15), V. mimicus (positions 16 and 17), V. alginolyticus (positions 18 and 19), V. vulnificus (positions 20 and 21), A. hydrophila/caviae (position 22), A. salmonicida (position 23), P. aeruginosa (position 24), S. putrefaciens (position 25), and E. coli O157:H7 (position 26). The no-template control (NTC) contained deionized water.