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Transcriptional activity in V. parahaemolyticus Vp5 in ASW at 4°C. Quantification of the mRNA copy numbers for the rpoS, fur, pvsA, pvuA, tdh2, escU, vopP, and spa24 genes was performed by real-time RT-PCR. The number of days necessary to decrease the mRNA population by half (T½) is indicated for each gene. Sample graphs depicting the reduction in the mRNA for a housekeeping gene (rpoS; panel A) and a virulence gene (tdh2; panel B) are shown.
