TABLE 2.
Detection of aryl-β-glucosidase activity from Lactobacillus delbrueckii mutant Uc-3 grown in different sugar substratesa
| Enzyme source | Substrate | Enzyme activity (U/g of cells) in:
|
|||
|---|---|---|---|---|---|
| Glucose | Lactose | Hydrolyzed sucrose | Cellobiose | ||
| Cells | pNPG | 2.2 ± 0.20 | 6.5 ± 0.55 | 1.5 ± 0.13 | 11.8 ± 1.0 |
| pNPgal | 4.1 ± 0.35 | 6.0 ± 0.50 | 4.0 ± 0.30 | 3.0 ± 0.25 | |
| pNPC | ND | 1.3 ± 0.12 | ND | 4.5 ± 0.30 | |
| Sonicated cells | pNPG | ND | 4.1 ± 0.35 | ND | 5.5 ± 0.45 |
| pNPgal | 1.0 ± 0.10 | 4.5 ± 0.30 | 1.3 ± 0.10 | 1.7 ± 0.15 | |
| pNPC | ND | 0.8 ± 0.07 | ND | 2.0 ± 0.18 | |
a
The cells were harvested at the onset of stationary phase for the determination of enzyme activity. Values are the averages of two independent experiments. ND, not detected.