FIG. 1.

Analysis of HexA and M6PHexA by SDS-PAGE (A) and Western blotting (B). Purified HexA and M6PHexA were separated by 10% SDS-PAGE and analyzed by CBB staining (A) and immunostaining (B). The blotted membrane was overlaid with rabbit anti-human HexA serum, followed by alkaline phosphatase-conjugated anti-rabbit immunoglobulin G as the secondary antibody. CDP-Star detection reagent (GE Healthcare Bio-Sciences Corp.) was used to visualize the enzymes. M, molecular marker; lane 1, HexA; lane 2, M6PHexA.