FIG. 2.
Primary structure and inner processing sites of the α- and β-subunits of human HexA. (A) EndoHf-treated HexA blotted onto PVDF membrane and stained with CBB. The EndoHf treatment was performed according to the manufacturer's instructions. The two major bands (α and β) were analyzed for their N-terminal sequences. The primary structures of the α-subunit (B) and the β-subunit (C) of HexA are shown. Asterisks show the N-terminal amino acid detected by protein sequencing. Signal sequences are shown by the arrows. Amino acids that are proteolytically processed in mammalian HexA are boxed.