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. 2007 Jun 8;73(15):4805–4812. doi: 10.1128/AEM.00463-07

FIG. 5.

FIG. 5.

Analysis of M6PHexA uptake by TS and SD fibroblasts. (A) TS and SD fibroblasts were cultured in medium containing recombinant HexA enzymes (600 nmol/h/well, toward MUGS), and the MUGS-hydrolyzing activity of homogenates was measured to determine enzyme incorporation. 1, no enzyme addition; 2, HexA; 3, M6PHexA; 4, M6PHexA with 5 mM M6P. Each bar represents the mean of two independent experiments. The cellular MUGS-hydrolyzing activity of fibroblasts from a normal subject was 752 nmol/h/mg. Error bars represent standard errors of the means. (B) Dose dependency of enzyme uptake was determined by the addition of M6PHexA to the TS and SD fibroblasts at the MUGS-hydrolyzing activities of 200, 600, and 1,800 nmol/h/well. The MUGS-hydrolyzing activity of cell extracts was determined to detect enzyme incorporation.