TABLE 2.
Analysis of HexA N-glycana
| Sugar | % of total
|
|
|---|---|---|
| Neutral (nonphosphorylated) | Acidic (phosphorylated)b | |
| Man8GlcNAc2 | 14.2 | 1.1 |
| Man9GlcNAc2 | 15.9 | 1.4 |
| Man10GlcNAc2 | 22.9 | 1.5 |
| Man11GlcNAc2 | 21.5 | 1.2 |
| Man12GlcNAc2 | 9.8 | 0.5 |
| Man13GlcNAc2 | 3.5 | 0.2 |
| Man14GlcNAc2 | 1.9 | 0.2 |
| ≥Man15GlcNAc2 | 4.2 | ND |
| Total | 93.9 | 6.1 |
a
Man, mannose; ND, not detected. n = 3. The values were calculated from the peak areas under the assay conditions for HPLC, as described in Materials and Methods.
b
The acidic sugars include mannose-6-phosphate.