TABLE 5.
Summary of distributions of nirK OTUs from samples FB064, FB067, and FB066 and nirS OTUs from samples FB064 and FB067
| OTU group and name | No. of clones per OTU
|
Closest cultured relative(s)b | % Similarityc | ||
|---|---|---|---|---|---|
| FB064 (stimulated) | FB067 (stimulated) | FB066a (control) | |||
| nirK OTUs | |||||
| 1K | 19 | 19 | 2 | FRC isolates 4.5A2 and 7.5A2 | 100.0 |
| 2K | 1 | 0 | 0 | Nitrosomonas sp. strain TA92liNH4 | 84.8 |
| 4K | 0 | 0 | 1 | Ochromobactrum sp. strain 4FB14 | 93.5 |
| 7K | 1 | 11 | 7 | Alcaligenes sp. strain DSM 30128 | 81.7 |
| 8K | 0 | 1 | 0 | FRC isolate 4.5A2 | 84.7 |
| 9K | 0 | 1 | 0 | FRC isolate 4.5A2 | 94.2 |
| 10K | 1 | 0 | 0 | FRC isolate 4.5A2 | 92.5 |
| 11K | 1 | 0 | 0 | FRC isolate 4.5A2 | 98.0 |
| 12K | 1 | 0 | 0 | FRC isolate 4.5A2 | 87.5 |
| 13K | 1 | 0 | 0 | FRC isolate 4.5A2 | 100.0 |
| nirS OTUs | |||||
| 1S | 38 | 0 | Dechloromonas aromatica | 90.5 | |
| 9S | 0 | 7 | Thiobacillus denitrificans | 75.9 | |
| 10S | 0 | 1 | Ralstonia eutropha | 75.1 | |
| 11S | 0 | 1 | Magnetospirillum magnetotacticum | 85.1 | |
| 14S | 0 | 10 | Ralstonia metallidurans | 78.5 | |
| 18S | 0 | 1 | Ralstonia eutropha | 82.2 | |
| 19S | 0 | 3 | Ralstonia eutropha | 82.7 | |
| 20S | 11 | 0 | Ralstonia metallidurans | 74.1 | |
| 21S | 6 | 0 | Azoarcus tolulyticus | 81.5 | |
| 22S | 1 | 0 | Azoarcus tolulyticus | 84.3 | |
| 23S | 1 | 0 | Azoarcus tolulyticus | 94.6 | |
| 24S | 1 | 0 | Azoarcus tolulyticus | 87.0 | |
| 25S | 1 | 0 | Azoarcus tolulyticus | 86.6 | |
| 26S | 0 | 2 | Ralstonia metallidurans | 84.4 | |
| 27S | 0 | 8 | Magnetospirillum magnetotacticum | 86.6 | |
| 28S | 0 | 3 | Magnetospirillum magnetotacticum | 82.8 | |
| 34S | 0 | 5 | Magnetospirillum magnetotacticum | 85.2 | |
| 35S | 4 | 0 | Magnetospirillum magnetotacticum | 87.9 | |
| 36S | 0 | 3 | Thauera aromatica | 80.4 | |
| Pseudomonas stutzeri | 80.4 | ||||
| 37S | 2 | 0 | Ralstonia metallidurans | 74.7 | |
| 38S | 0 | 10 | Dechloromonas aromatica | 88.8 | |
| 39S | 0 | 10 | Dechloromonas aromatica | 87.8 | |
| 41S | 0 | 3 | Dechloromonas aromatica | 89.8 | |
| 42S | 0 | 1 | Dechloromonas aromatica | 91.9 | |
| 43S | 1 | 0 | Dechloromonas aromatica | 96.6 | |
| 44S | 0 | 2 | Dechloromonas aromatica | 88.8 | |
a
An amplified PCR product using nirS primers could not be obtained from this sample.
b
GenBank accession numbers for closest cultured relatives are located next to corresponding genus and species names in Fig. 4 and 5.
c
Similarity values are based on pair-wise distance values from multiple alignment files using translated amino acid sequences.