. 2007 Jun 8;73(15):4769–4775. doi: 10.1128/AEM.00873-07

TABLE 2.

PCR/RFLP typing of Stx1 family genes with BstEII, HaeII, and PflMI

Restriction enzyme	Size(s) (bp) of restriction fragment(s)^a
Restriction enzyme	stx₁^b	stx_1c^c	stx_1d^d
BstEII	282^e	224, 59	282^e
HaeII	282^e	283^e	202, 80
PflMI	174, 108	283^e	282^e

Fragments were obtained by enzymatic digestion of PCR products obtained with primers Stx1com-F and Stx1com-R.

Results for stx₁ (GenBank accession no. M16625), stx_1-O48 (GenBank accession no. Z36899), and stx_1-CB168 (GenBank accession no. Z36900).

Results for stx_1c (GenBank accession no. AJ413986 and Z36901).

Results for stx_1d (GenBank accession no. AY170851 and AY986980).

Undigested.