. 2007 Jun 8;73(15):4769–4775. doi: 10.1128/AEM.00873-07

TABLE 3.

PCR/RFLP typing of Stx2 family genes with HaeIII, RsaI, FokI, and NciI

Restriction enzyme	Size(s) (bp) of restriction fragment(s)^a
Restriction enzyme	stx₂^b	stx_2v-ha^c	stx_2v-hb^d	stx_2g^e	stx_2-NV206^f	stx_2-EC1586^g
HaeIII	269^h	142, 127	142, 127	269^h	269^h	269^h
RsaI	219, 50	139, 80, 50	219, 50	269^h	219, 50	219, 50
FokI	154, 115	269^h	269^h	269^h	269^h	269^h
NciI	269^h	269^h	140, 129	269^h	140, 129	269^h

Fragments were obtained by enzymatic digestion of PCR products obtained with primers GK5 and GK6.

GenBank accession no. X07865 and AP000422.

GenBank accession no. AJ605767 and AF879828.1.

GenBank accession no. AF479829.2, AF500190, and AF500191.

GenBank accession no. AY286000 and AJ966783.

GenBank accession no. AF329817, AF500187, AF500197, and AY095209.

GenBank accession no. AM498375 and AF500188.

Undigested.