TABLE 1.
Specificity of IS2404, IS2606, and KR real-time PCR assays
| Mycobacterial species (strain) | Origin | Sourceb | IS2404 | IS2606 | KR |
|---|---|---|---|---|---|
| M. ulcerans (ATCC 19423) | Victoria, Australia | VIDRL | +c | + | + |
| M. ulcerans (63/04) | Victoria, Australia | VIDRL | + | + | + |
| M. ulcerans (40/02) | Western Australia | VIDRL | + | + | + |
| M. ulcerans (75/04) | Northern Territory, Australia | + | + | + | |
| M. ulcerans (20213/91) | Queensland, Australia | VIDRL | + | + | + |
| M. ulcerans (38/02) | Queensland, Australia | VIDRL | + | + | + |
| M. ulcerans (71/05) | Papua New Guinea | VIDRL | + | + | + |
| M. ulcerans (5145) | Zaire | ITM | + | + | + |
| M. ulcerans (98912) | People's Republic of China | ITM | + | + | + |
| M. ulcerans (842) | Suriname | ITM | + | + | + |
| M. ulcerans (9118) | Mexico | ITM | + | + | + |
| M. ulcerans (186510) | Malaysia | VIDRL | + | + | + |
| M. liflandii (128FXT) | California | UTK | + | + | + |
| M. pseudoshottsii (L15) | Chesapeake Bay, United States | VIMS | + | + | + |
| M. marinum (DL045)a | Mediterranean Sea, Greece | NCM | + | + | + |
| M. marinum (CC240299)a | Fresh water, Israel | NCM | + | NDd | + |
| M. marinum (DL240490)a | Red Sea, Israel | NCM | + | + | + |
| M. tuberculosis (H37Rv) | Sequence strain | VIDRL | ND | ND | ND |
| M. paratuberculosis (field strain) | VIDRL | ND | ND | ND | |
| M. marinum (NCTC 2275) | VIDRL | ND | ND | ND | |
| M. marinum (ITM 00-1026) | France | IP | ND | ND | ND |
| M. kansasii (NCTC 10268) | VIDRL | ND | ND | ND | |
| M. xenopi (NCTC 10042) | VIDRL | ND | ND | ND | |
| M. szulgai (NCTC 10831) | VIDRL | ND | ND | ND | |
| M. simiae (ATCC 25275) | VIDRL | ND | ND | ND | |
| M. marinum (NCTC 2275) | VIDRL | ND | ND | ND | |
| M. malmoense (NCTC 11298) | VIDRL | ND | ND | ND | |
| M. asiaticum (21674) | VIDRL | ND | ND | ND | |
| M. avium (ATCC 25291) | VIDRL | ND | ND | ND | |
| M. intracellulare (ATCC 13950) | VIDRL | ND | ND | ND | |
| M. lentiflavum (A) | Patient isolate | VIDRL | ND | ND | ND |
| M. lentiflavum (B) | Patient isolate | VIDRL | ND | ND | ND |
| M. lentiflavum (C) | Patient isolate | VIDRL | ND | ND | ND |
a
These isolates were originally designated M. marinum because of their growth characteristics, but they have since been shown to produce mycolactone and are now described as “other mycolactone-producing mycobacteria” (18, 32).
b
IP, Institut Pasteur, Paris, France; ITM, Institute for Tropical Medicine, Antwerp, Belgium; NCM, National Centre for Mariculture, Eilat, Israel; UTK, Department of Microbiology, University of Tennessee, Knoxville, TN; VIDRL, Victorian Infectious Diseases Reference Laboratory, Victoria, Australia; VIMS, Virginia Institute of Marine Science, Gloucester Point, VA.
c
+, presence of.
d
ND, not detected.