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RT-PCR analysis of wild-type HD100 and the strB mutant. Total RNA was extracted from E. coli ML35 (lane 1), wild-type HD100 (lane 2), and HD100ΔstrB (lane 3) and used in RT-PCRs with primers specific for the strB gene. The rpsL gene was used as a load control for B. bacteriovorus RNA. The absence of an RT-PCR product in lane 1 indicates that the product seen for both the strB and the rpsL genes corresponds to B. bacteriovorus RNA rather than any possible contaminating E. coli RNA.
