TABLE A1.
Enzyme activities in diauxic shift cellsa
| Metabolic pathway | Enzyme | Activity (U g protein−1) (mean ± SD) |
|---|---|---|
| Glycolysis | Hexokinaseb | 1,580 ± 200 |
| Phosphoglucose isomerase | 1,960 ± 110 | |
| Phosphofructokinase | 250 ± 40 | |
| Aldolase | 420 ± 30 | |
| Triose phosphate isomerase | 3,570 ± 440 | |
| Glyceraldehyde phosphate dehydrogenase | 4,750 ± 230 | |
| Phosphoglycerate kinase | 7,840 ± 390 | |
| Phosphoglycerate mutase | 1,070 ± 700 | |
| Enolase | 1,840 ± 120 | |
| Pyruvate kinase | 3,060 ± 250 | |
| Ethanol and acetate branch | Pyruvate decarboxylase | 1,680 ± 120 |
| Alcohol dehydrogenase | 2,650 ± 380 | |
| NAD-aldehyde dehydrogenase | 19 ± 2 | |
| NADP-aldehyde dehydrogenase | 55 ± 4 | |
| Glycerol branch | Glycerol-3-phosphate dehydrogenase | 184 ± 31 |
| Glycerol-3-phosphate phosphatase | 106 ± 18 | |
| Others | Glucose-6-phosphate dehydrogenase | 115 ± 7 |
| Phosphoenolpyruvate carboxykinase | 118 ± 9 |
a
Means were determined using two to four independently grown cultures, and the mean standard deviations were estimated using also the data for starved cells shown in Fig. 1. Protein contents were determined using the method of Lowry et al. (24).
b
The ratio of the activity with fructose as the substrate to the activity with glucose as the substrate was 1.62.