FIG. 3.

Effects of various interface PACVEIRs on the standard deviation of biovolume and mean thickness of BD413 biofilms Ac1 to Ac4 (A to D, respectively), JMP228n::gfp biofilms Ral1 and Ral2 (E and F, respectively), and JMP228n::gfp biofilms Jmp1 to Jmp4 (G to J, respectively). The percent area coverage criterion for the substratum was held constant at 1%. The vertical dashed line indicates the maximum standard deviation of mean thickness and the corresponding optimal PACVEIR. Utilizing the identified percent area coverage value as the criterion for image removal maintains the inherent structural variation of the biofilm and sets a threshold value that is both unbiased and reproducible. BD413 biofilms (A to D) had a characteristic PACVEIR that was nearly 0%, while JMP228n::gfp biofilms (E to J) had an average interface PACVEIR near 0.1%. The x axes are not to scale.