TABLE 1.
Immunization schedule for the different forms of vaccines
| Group | Vaccine | Route of administration | Dose per mouse |
|---|---|---|---|
| 1 | pcDNA-S | i.m. | 200 μg |
| 2 | pcDNA-S + pcDNA-IL-2 | i.m. | 200 + 50 μg |
| 3 | pcDNA-S | i.e.a | 30 μg |
| 4 | pcDNA-S + pcDNA-IL-2 | i.e. | 30 + 8 μg |
| 5 | pcDNA-S | Oralb | 5 × 109 CFU |
| 6 | pcDNA-S + pcDNA-IL-2 | Oral | 5 × 109 CFU + 1 × 109 CFU |
| 7 | pcDNA3.1 | i.m. | 200 μg |
| 8 | pcDNA-IL-2 | i.m. | 200 μg |
| 9 | PBS | i.m. | 100 μl |
| 10 | pcDNA3.1 | Oral | 5 × 109 CFU |
| 11 | CS022 | Oral | 5 × 109 CFU |
a
i.e., electroporation.
b
Oral immunization with attenuated S. enterica serovar Typhimurium CS022.