TABLE 1.
Strain | Descriptiona |
---|---|
BCG-P | BCG-P parent |
222empPas | Empty vector pJH22 in BCG-P |
120surPas | CON6 gp120 for surface form in BCG-P |
120intPas | CON6 gp120 for intracellular form in BCG-P |
120secPas | CON6 gp120 for secreted form in BCG-P |
140surPas | CON6 gp140CF for surface form in BCG-P |
140intPas | CON6 gp140CF for intracellular form in BCG-P |
140secPas | CON6 gp140CF for secreted form in BCG-P |
BCG-D | BCG-D parent |
222empDan | Empty vector pJH22 in BCG-D |
120surDan | CON6 gp120 for surface form in BCG-D |
120intDan | CON6 gp120 for intracellular form in BCG-D |
120secDan | CON6 gp120 for secreted form in BCG-D |
140surDan | CON6 gp140CF for surface form in BCG-D |
140intDan | CON6 gp140CF for intracellular form in BCG-D |
140secDan | CON6 gp140CF for secreted form in BCG-D |
All constructs have a kanamycin resistance gene for selection and were cloned into ApaI and HpaI restriction endonuclease sites in vectors. C, cleavage site deletion; F, fusion domain deletion. All gp140 constructs have the transmembrane and cytoplasmic domains deleted.