TABLE 1.
Recombinant BCG strains generated for expression of HIV-1 envelope
| Strain | Descriptiona |
|---|---|
| BCG-P | BCG-P parent |
| 222empPas | Empty vector pJH22 in BCG-P |
| 120surPas | CON6 gp120 for surface form in BCG-P |
| 120intPas | CON6 gp120 for intracellular form in BCG-P |
| 120secPas | CON6 gp120 for secreted form in BCG-P |
| 140surPas | CON6 gp140CF for surface form in BCG-P |
| 140intPas | CON6 gp140CF for intracellular form in BCG-P |
| 140secPas | CON6 gp140CF for secreted form in BCG-P |
| BCG-D | BCG-D parent |
| 222empDan | Empty vector pJH22 in BCG-D |
| 120surDan | CON6 gp120 for surface form in BCG-D |
| 120intDan | CON6 gp120 for intracellular form in BCG-D |
| 120secDan | CON6 gp120 for secreted form in BCG-D |
| 140surDan | CON6 gp140CF for surface form in BCG-D |
| 140intDan | CON6 gp140CF for intracellular form in BCG-D |
| 140secDan | CON6 gp140CF for secreted form in BCG-D |
a
All constructs have a kanamycin resistance gene for selection and were cloned into ApaI and HpaI restriction endonuclease sites in vectors. C, cleavage site deletion; F, fusion domain deletion. All gp140 constructs have the transmembrane and cytoplasmic domains deleted.